The icon depics three Orthodox Saints: Saint Melania the Younger, Saint Cosmas the Unmercenary Healer, and Saint Alexios Man of God.
Saint Melania the Younger is a Christian saint and Desert Mother who lived during the reign of Emperor Honorius, son of Theodosius I. She is the paternal granddaughter of Melania the Elder. The Feast of Melania the Younger is held on 31 December.
Saint Cosmas (also written Kosmas) (died ca. 287) was a physician, and early Christian martyr born in Cilicia, part of today's Turkey. He, with his twon brother Damianus, practiced his profession in the seaport of Ayas, Adana, then in the Roman province of Syria. Accepting no payment for their services led to them being named "Ανάργυροι" (Unmercenary); it has been said that, by this, they attracted many to the Christian faith. During the persecution under Diocletian, Cosmas and Damian were arrested by order of the Prefect of Cilicia, who ordered them under torture to recant. However, according to legend they stayed true to their faith, enduring being hung on a cross, stoned and shot by arrows and finally suffered execution by beheading.
Saint Alexios the Holy Man was a fourth-century monastic who is known for his dedication to Christ in anonymity. He was born in Rome to a wealthy family, but lived in Edessa, as a beggar, and shared the alms he received with other poor people. A miraculous vision of the Blessed Virgin Mary singled him out as a "Man of God." His feast day is commemorated by the Church on March 17.